Catalogue 2018
For fast amplification of complex targets Xpert Fast Hotstart DNA polymerase is a robust enzyme, suitable for the amplification of difficult targets with extreme speed (4-8kb/min), yield and specificity.

Main application: fast amplification of complex targets

Xpert Fast Hotstart DNA polymerase is a robust enzyme, suitable for the amplification of difficult targets with extreme speed (4-8kb/min), yield and specificity.
The buffer composition has been optimized via high-throughput screening and makes the enzyme particularly resistant to PCR inhibitors. As a result, Xpert Fast Hotstart DNA Polymerase is suitable for direct PCR from unpurified samples, including blood, urine, and bacterial colonies, making this the ideal choice for consistent results in fast complex PCR amplifications.

Performance highlights:
- Fast amplification of complex targets (including GC/AT-rich)
- Direct PCR from crude samples

Properties:
Amplicon size:  up to 5kb
Extension Rate: 4-8 kb/min
Hotstart:           Yes
A-overhang:      Yes

Applications:  
- Direct PCR
- Fast amplification of complex targets (including GC-rich and AT-rich templates)
- Colony PCR
- Multiplex PCR

Components:
- Kit version with dNTPs included (in the buffer)
- Mastermix available with (red) dye, and without dye

Storage:-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.

Product Info - Xpert Fast Hotstart DNA Polymerase (with dNTPs) Download
Product Info - Xpert Fast Hotstart Mastermix Download
Product Info - Xpert Fast Hotstart Mastermix with dye Download
Reference Description Quantity Price
GE25.0250 Xpert Fast Hotstart DNA Polymerase (with dNTPs) 250U
GE35.0001 Xpert Fast Hotstart 2X Mastermix (80rxn) 1mL
GE35.5001 Xpert Fast Hotstart 2X Mastermix (400rxn) 5x 1mL
GE45.0001 Xpert Fast Hotstart 2X Mastermix with Dye (80rxn) 1mL
GE45.5001 Xpert Fast Hotstart 2X Mastermix with Dye (400rxn) 5x 1mL