Main application: fast amplification of complex targets
Xpert Fast Hotstart DNA polymerase is a robust enzyme, suitable for the amplification of difficult targets with extreme speed (4-8kb/min), yield and specificity.
The buffer composition has been optimized via high-throughput screening and makes the enzyme particularly resistant to PCR inhibitors. As a result, Xpert Fast Hotstart DNA Polymerase is suitable for direct PCR from unpurified samples, including blood, urine, and bacterial colonies, making this the ideal choice for consistent results in fast complex PCR amplifications.
- Fast amplification of complex targets (including GC/AT-rich)
- Direct PCR from crude samples
Amplicon size: up to 5kb
Extension Rate: 4-8 kb/min
- Direct PCR
- Fast amplification of complex targets (including GC-rich and AT-rich templates)
- Colony PCR
- Multiplex PCR
- Kit version with dNTPs included (in the buffer)
- Mastermix available with (red) dye, and without dye
Storage:-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.
|Product Info - Xpert Fast Hotstart DNA Polymerase (with dNTPs)||Download|
|Product Info - Xpert Fast Hotstart Mastermix||Download|
|Product Info - Xpert Fast Hotstart Mastermix with dye||Download|
|GE25.0250||Xpert Fast Hotstart DNA Polymerase (with dNTPs)||250U|
|GE35.0001||Xpert Fast Hotstart 2X Mastermix (80rxn)||1mL|
|GE35.5001||Xpert Fast Hotstart 2X Mastermix (400rxn)||5x 1mL|
|GE45.0001||Xpert Fast Hotstart 2X Mastermix with Dye (80rxn)||1mL|
|GE45.5001||Xpert Fast Hotstart 2X Mastermix with Dye (400rxn)||5x 1mL|