Ready-to-use three-colour protein standard suitable for monitoring protein separation during SDS-PAGE, size determination of proteins, and verification of transfer efficiency of Western Blotting onto membrane (compatible with PVDF, nylon and nitrocellulose). This marker is composed of 10 pre-stained proteins ranging from 6,5kDa to 270kDa. For easy identification of each band, it includes three reference bands of different colours that co-migrate with proteins of 30kDa (red), 52kDa (green) and 270kDa (red), respectively, when separated by 4-20% gradient SDS-PAGE using TGS Buffer.
Quantity: 500μl of approx. 0.1~0.4 mg/ml of each protein in 20mM Tris-phosphate , pH 7.5 (25°C), 2% SDS, 1mM Dithiothreitol, 3.6M Urea, and 15% (v/v) Glycerol.
Storage: Up to 2 weeks at room temperature, 3 months at +4ºC, 2 years at -20ºC.
6.5 | 16 | 30 | 37 | 52 | 66 | 95 | 130 | 175 | 270 (Tris-Glycine 4-20%)
3μl or 5μl for clear visualization during electrophoresis on mini-gel (100-150 lanes).
1.5~2.5 μl per well for general Western transferring (200-300 lanes).
Apply more for thicker (> 1.5 mm) or larger gels.
|Product Info GRS Protein Marker MulitColour Plus v||Download|
|GLP03.0500||GRS Protein Marker MultiColour PLUS (100 lanes)||500uL|
|GLP03.3500||GRS Protein Marker MultiColour PLUS (300 lanes)||3x 500uL|